Journal article
Phenotypic and functional characterization of pharmacologically expanded Vγ9Vδ2 T cells in pigtail macaques
IM Barber-Axthelm, KM Wragg, R Esterbauer, TH Amarasena, VRB Barber-Axthelm, AK Wheatley, AM Gibbon, SJ Kent, JA Juno
Iscience | Published : 2023
Abstract
While gaining interest as treatment for cancer and infectious disease, the clinical efficacy of Vγ9Vδ2 T cell-based immunotherapeutics has to date been limited. An improved understanding of γδ T cell heterogeneity across lymphoid and non-lymphoid tissues, before and after pharmacological expansion, is required. Here, we describe the phenotype and tissue distribution of Vγ9Vδ2 T cells at steady state and following in vivo pharmacological expansion in pigtail macaques. Intravenous phosphoantigen administration with subcutaneous rhIL-2 drove robust expansion of Vγ9Vδ2 T cells in blood and pulmonary mucosa, while expansion was confined to the pulmonary mucosa following intratracheal antigen admi..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
The authors would like to acknowledge and thank the Melbourne Cytometry Platform for provision of flow cytometry services, and Vanta Jameson and Magdaline Sakkas from the Melbourne Brain Center Flow Cy- tometry Facility for provision of sorting services. All nonhuman primate studies were conducted at the Mon-ash Animal Research Platform Gippsland Field Station. We acknowledge and thank Irwin Ryan, Graham Shillito, and staff for provision of animal care and sample collection support throughout the animal trials. The study was funded by the NHMRC through Program (1149990) and Investigator grants (A.K.W., GNT1173433; J.A.J., GNT2009308) . I.M.B.A. is supported by a Melbourne Research Scholarship through the University of Melbourne.